3.85, 508 Touro, NYIT, PCOM

Is it worth applying to the following...
Touro NY
NYIT
PCOM
3.85 GPA, 508 MCAT
Above average ECs
NJ resident

I have shadowed DOs, but my letter of rec within my committee letter is an MD

When is the latest that I can apply and still be competitive to these programs?

I've applied to 27 MD schools. I just want to maximize my chances at an acceptance this cycle. But I don't want to waste a decent amount of money applying to DO schools if I won't have a chance without a DO letter of rec